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Citation Information

Stock #: JFS0309
Volume: 48
Issue: 5
Year: 2003
Pages: 1088-1090
Author(s): Iida R, Sawazaki K, Ikeda H, Miyamoto T, Tsubota E, Takatsuka H, Masuyama M, Matsuki T, Yasudd T, Kishi K
Title: A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445
Keywords: forensic science


Abstract: We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs—DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 mL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.

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