The Development of DNA Profiling Database in an HAE III Based RFLP System for Chinese, Malays, and Indians in Singapore
Chow, ST
Scientific officer, Department of Scientific Services, Institute of Science and Forensic Medicine,Singapore.

Tan, WF
Scientific officer, Department of Scientific Services, Institute of Science and Forensic Medicine,Singapore.

Yap, KH
Technical officer, Department of Scientific Services, Institute of Science and Forensic Medicine,Singapore.

Ng, TL
Director, Department of Scientific Services, Institute of Science and Forensic Medicine, Singapore.


Abstract
Deoxyribonucleic acid (DNA) restriction fragment length polymorphism (RFLP) profiles were obtained for blood specimens from the three population groups—Chinese, Malays and Indians—in Singapore. The population databases were collected from Hae III digested high molecular weight DNA hybridized with four variable number of tandem repeats (VNTR) loci - D2S44, D10S28, D4S139 and D1S7. The data were analyzed statistically using the fixed bin system. Comparison of ratio of bin frequencies of these population data with published data on whites, blacks, and hispanics shows that the alleleic distribution at these loci is not seriously different among the six groups. This has important implications to the statistical significance of forensic DNA applications.

Keywords:
allele frequency, binning system, Chinese, deoxyribonucleic acid (DNA), DNA, forensic science, genetic typing, Indians, Malays, paternity index, pathology and biology, population database, restriction fragment length polymorphism, restriction fragment length polymorphism (RFLP), variable number of tandem repeats (VNTR)

Paper ID: JFS384930874

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Author Chow ST, Tan WF, Yap KH, Ng TL Title The Development of DNA Profiling Database in an HAE III Based RFLP System for Chinese, Malays, and Indians in Singapore Symposium , Committee on