Simultaneous Gas Chromatography/Mass Spectrometry Assay of Methadone and 2-Ethyl-1,5-Dimethyl-3,3-diphenyl-pyrrolidine (EDDP) in Urine
Baugh, LD
Former graduate student and professor, respectively, Department of Criminal Justice, University of Alabama at Birmingham, AL.

Liu, RH
Former graduate student and professor, respectively, Department of Criminal Justice, University of Alabama at Birmingham, AL.

Walia, AS
Chief, Alcoholism Research, VA Medical Center, and research associate professor. Department of Surgery, School of Medicine, University of Alabama at Birmingham. AL.


Abstract
An efficient extraction and gas chromatography/mass spectrometry (GC/MS) procedure has been developed for the simultaneous determination of methadone and 2-ethyl-1, 5-dimethyl-3,3-diphenylpyrrolidine in urine samples. The merits of this procedure include (1) effective high-volume sample processing; (2) excellent gas chromatography characteristics; (3) high precision for quantitative methadone determination—1.0% coefficient of variation (CV) for GC/MS injection replicates and 1.2% for extraction replicates; (4) excellent linearity within the range (0 to 1200 ng/mL) studied; and (5) adequate detection limits (50 ng/mL) for most practical purposes. The detection limit for methadone may be improved 40-fold by using a different internal standard.

Keywords:
2-ethyl-1,5-dimethyl-3,3-diphenylpyrrolidine, 2-ethyl-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), abuse drugs, chemical analyses, chemical analysis, forensic science, GC/MS, methadone, selected ion monitoring, toxicology, urinalysis, urine

Paper ID: JFS362910548

ASTM International is a member of CrossRef.
Author Baugh LD, Liu RH, Walia AS Title Simultaneous Gas Chromatography/Mass Spectrometry Assay of Methadone and 2-Ethyl-1,5-Dimethyl-3,3-diphenyl-pyrrolidine (EDDP) in Urine Symposium , Committee on