Capillary Gas-Liquid Chromatography Separation of Phenethylamines in Amphetamine-Positive Urine Samples
DePace, A
Research scientist, New York State Psychiatric Institute, New York, NY.

Verebey, K
Director of toxicology, New York City Department of Health, New York, NY, and associate professor of psychiatry, State University of New York Health Science Center, Brooklyn, NY.

ElSohly, M
Director of EISohly Laboratories, Inc., Oxford, MS.


Abstract
Good gas chromatography (GC) separation of molecules is essential for clean gas chromatography/mass spectrometry (GC/MS) confirmation of compounds. The trifluoro derivatives of ephedrine (E) and methamphetamine (MA) coelute on dimethyl silicone capillary columns, such as DB-1, which are most commonly used by chromatographers. Methods are described to separate E and MA to aid GC/MS confirmations of methamphetamine, ephedrine, or both E and MA together, whichever may be present in Enzyme Immunoassay (EIA)-analyzed amphetamine-positive urine samples. The use of the heptafluoro derivatives of E and MA on a DB-1 column, or the trifluoro derivatives of E and MA on a DB-17 column, is suggested for good gas chromatographic separation.

Keywords:
chromatographic separation, ephedrine, forensic science, immunoassay, methamphetamine, toxicology, urine

Paper ID: JFS356901431

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Author DePace A, Verebey K, ElSohly M Title Capillary Gas-Liquid Chromatography Separation of Phenethylamines in Amphetamine-Positive Urine Samples Symposium , Committee on