Chemically Sensitized Erythrocytes for Hemagglutination Reactions
Harrington, JJ
Senior forensic scientist, New Jersey State Police, North Regional Laboratory, Little Falls, NJ.

Gordon, RE
Associate professor, Pathology Department, Mount Sinai School of Medicine, New York, NY.

Ross, JP
Visiting investigator, John Jay College of Criminal Justice, City University of New York, New York, NY.

Kobilinsky, L
Professor, Department of Forensic Science, John Jay College of Criminal Justice, and member, Doctoral Faculty Biochemistry, City University Graduate Center, City University of New York, New York, NY.


Abstract
The use of chemically modified indicator erythrocytes for hemagglutination reactions can result in increased sensitivity. Treatment of erythrocytes with polyvinylpyrrolidone (PVP) or dextran T40 (10% weight/volume) induces changes in the cell surface in the form of extensions and blebbing, thereby increasing the surface area. These sensitized cells can be used in forensic science when detection or quantitation of erythrocyte surface reacting antibodies is important. The effect of altering membrane lipid fluidity on erythrocyte surface antigens has also been investigated. Treatment of cells with a reagent that increases the membrane ratio of cholesterol to phospholipid results in enhanced hemagglutination capacity despite the lack of extensive spiculation.

Keywords:
antibody, antigen, bovine serum albumen (BSA), dextran, erythrocytes, forensic science, forensic serology, hemagglutination, polymer, polyvinylpyrrolidone (PVP), serology, zeta potential

Paper ID: JFS355901115

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Author Harrington JJ, Gordon RE, Ross JP, Kobilinsky L Title Chemically Sensitized Erythrocytes for Hemagglutination Reactions Symposium , Committee on