A new allele of the short tandem repeat (STR) locus, CSF1PO
Margolis-Nunno, H
Science Department, John Jay College of Criminal Justice, City University of New York, New York, N.Y.

Brenner, L
DNA Identification Laboratory, Philadelphia Police Department, Philadelphia, PA.

Cascardi, J
Science Department, John Jay College of Criminal Justice, City University of New York, New York, N.Y.

Kobilinsky, L
Science Department, John Jay College of Criminal Justice, City University of New York, New York, N.Y.


Abstract
CSF1PO is one of the thirteen core loci used for the CODIS database, and alleles reported for this short tandem repeat (STR) locus contain from 6 to 15 repeats of the tetranucleotide AGAT. Screening of DNA from 76 individuals by gel electrophoresis and silver stain detection yielded one sample that contained a rare, off-ladder CSF1PO allele; an allele larger than CSF1PO₁₅ was detected in a heterozygote that also contained a CSF1PO₁₀ allele. Capillary electrophoresis analysis using Gene-Scan™ software demonstrated that the variant allele contained four bases more than CSF1PO₁₅. Following agarose gel electrophoresis to separate the two alleles of the heterozygote and cycle sequencing using dye terminators, sequence analysis showed that the variant, which was otherwise identical to the CSF1PO GenBank sequence, contained exactly 16 AGAT repeats. These results demonstrate the existence of an additional CSF1PO allele, a previously unreported size variant, CSF1PO₁₆.

Keywords:
alleles, CSF1PO, DNA typing, forensic science, short tandem repeat, variants

Paper ID: JFS4661480

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Author Margolis-Nunno H, Brenner L, Cascardi J, Kobilinsky L Title A new allele of the short tandem repeat (STR) locus, CSF1PO Symposium , Committee on