Recovery of DNA for forensic analysis from lip cosmetics
Webb, LG
Forensic Biology Laboratory, Forensic Services, PathCentre, Hospital Avenue, Nedlands, Perth, Western Australia 6009, Australia.

Egan, SE
Forensic Biology Laboratory, Forensic Services, PathCentre, Hospital Avenue, Nedlands, Perth, Western Australia 6009, Australia.

Turbett, GR
Forensic Biology Laboratory, Forensic Services, PathCentre, Hospital Avenue, Nedlands, Perth, Western Australia 6009, Australia.


Abstract
To obtain a reference DNA profile from a missing person, we analyzed a variety of personal effects, including two lip cosmetics, both of which gave full DNA profiles. Further investigations were undertaken to explore this previously unreported source of DNA. We have tested a range of brands and types of lip cosmetics. Our studies have revealed that lip cosmetics are an excellent source of DNA, with almost 80% of samples giving a result. However, artifacts are frequently observed in the DNA profiles when Chelex is used for the DNA extraction and additional DNA purification procedures are required to ensure that an accurate DNA profile is obtained.

Keywords:
amelogenin, cosmetic, D13S317, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, DNA extraction, DNA typing, FGA, forensic science, human identification, lipstick, polymerase chain reaction, Profiler Plus, short tandem repeat, vWA

Paper ID: JFS4661474

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Author Webb LG, Egan SE, Turbett GR Title Recovery of DNA for forensic analysis from lip cosmetics Symposium , Committee on