PCR-based forensic testing of DNA from stained cytological smears
Dimo-Simonin, N
Institut Universitaire de Médecine Légale, Rue du Bugnon 21, Lausanne,Switzerland.

Grange, F
Institut Universitaire de Médecine Légale, Rue du Bugnon 21, Lausanne,Switzerland.

Brandt-Casadevall, C
Institut Universitaire de Médecine Légale, Rue du Bugnon 21, Lausanne,Switzerland.


Abstract
Experiments were performed to evaluate the efficiency of PCR-STR (Short Tandem Repeats) and PCR-sequence polymorphisms for the identification of stained pap smears and postcoital slides stained with cytological and forensic techniques. HLA-DQA1, PolyMarker, Amelogenin, HUMTH01, HUMV-WFA31, HUMF13B, and HUMFES/FPS were determined. With the exception of the forensic Baecchi stain, all the PCR-systems gave consistent results in comparison with the reference blood from the donors. Cytological stained smears can be important evidence for identification in sexual assault cases and in missing person cases.

Keywords:
amelogenin, cytology slides, D758, DNA, DNA typing, F13B, FES/FPS, forensic science, GC, genetic typing, GYPA, HBGG, histological stains, HLA-DQA1, LDLR, Pap smears, pathology and biology, polymerase chain reaction, polymerase chain reaction (PCR), sexual assault evidence, short tandem repeat, short tandem repeats, TH01, VWFA31

Paper ID: JFS423970506

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Author Dimo-Simonin N, Grange F, Brandt-Casadevall C Title PCR-based forensic testing of DNA from stained cytological smears Symposium , Committee on