Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation
Vincent, S
Department of Biology, head, Department of Entomology, and head, Department of Biology, respectively, Institut de Recherche Crimenelle de la Gendarmerie National, Rosny Sous Bois, France.

Vian, JM
Department of Biology, head, Department of Entomology, and head, Department of Biology, respectively, Institut de Recherche Crimenelle de la Gendarmerie National, Rosny Sous Bois, France.

Carlotti, MP
Department of Biology, head, Department of Entomology, and head, Department of Biology, respectively, Institut de Recherche Crimenelle de la Gendarmerie National, Rosny Sous Bois, France.


Abstract
The identification of insects found on a dead body can lead to the estimation of the time of death (postmortem interval). We report an updated version of an established method based on sequence analysis of PCR products from a region of the cytochrome b oxidase subunit I mitochondrial gene of different members of the family Calliphoridae, by sequencing six European species: Lucilia sericata (Meigen), Lucilia caesar (Linné), Lucilia illustris (Meigen), Calliphora vicina (Robineau-Desvody), Calliphora vomitoria (Linné;), Protophormia terraenovae (Robineau-Desvody) and one Guianese species: Cochliomyia macellaria (Fabricius). This technique provided clear results when applied to the larvae and we also report the identification of empty puparia.

Keywords:
blow flies, Calliphoridae, cytochrome oxydase gene, forensic science, mitochondrial DNA, polymerase chain reaction, postmortem interval, puparia, sequencing

Paper ID: JFS4540820

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Author Vincent S, Vian JM, Carlotti MP Title Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation Symposium , Committee on