Gelatinase A in human dentin as a new biochemical marker for age estimation
Martín-de las Heras, S
Assistant professor and associate professor, Department of Forensic Medicine and Forensic Odontology, University of Granada, 18071 Granada, Spain.

Valenzuela, A
Assistant professor and associate professor, Department of Forensic Medicine and Forensic Odontology, University of Granada, 18071 Granada, Spain.

Overall, CM
Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia V6T 1Z3 Vancouver, Canasa


Abstract
Dentin proteins from 24 individual permanent molars from patients aged 15 to 73 years were sequentially extracted, with guanidinium chloride (G1-extract) and then with EDTA; after demineralization, the material was again extracted with guanidinium chloride (G2-extract). Extracts were analyzed by SDS-PAGE electrophoresis and the gels were processed for zymography to detect gelatinolytic activities. The patterns of gelatinase A distribution differed in the different dentin protein fractions, and the changes varied with age. Significant differences were detected in gelatinase A in G2-extracts between individual younger than 20 years old and the rest of the sample (c²exp = 19.429; 1 d.f.; p £ 0.001). The incidence of true and false positives and negatives, and sensitivity and specificity for the presence of gelatinase A in dentin extracts, were calculated. Determination of gelatinase A in human dentin may be a useful marker to estimate age, especially when other morphological methods are of limited usefulness.

Keywords:
age, dentin proteins, forensic anthropology, forensic odontology, forensic science, gelatinase A, human dentin, matrix metalloproteinase, new biochemical marker

Paper ID: JFS4540807

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Author LaPorte, GM Affiliation United States Secret Service, Forensic Services Division, Questioned Document
Branch, 950 H Street NW, Washington, DC. Author Wilson, JD Affiliation United States Secret Service, Forensic Services Division, Questioned Document,
chemist contractor, 950 H Street NW, Washington, DC. Author Cantu, AA Affiliation United States Secret Service, Forensic Services Division, Research Section,
950 H Street NW, Washington, DC. Author Mancke, SA Affiliation United States Secret Service, Forensic Services Division, Questioned Document, chemist contractor, 950 H Street NW, Washington, DC. Author Fortunato, SL Affiliation
United States Secret Service, Forensic Services Division, Questioned Document
Branch, 950 H Street NW, Washington, DC. Author Martín-de las Heras S, Valenzuela A, Overall CM Title Gelatinase A in human dentin as a new biochemical marker for age estimation Symposium , Committee on