Usefulness of a toothbrush as a source of evidential DNA for typing
Tanaka, M
Postgraduate Student, Research Students, Associate Professor, and Professor, respectively, Department of Legal Medicine and Bioethics, Postgraduate School of Medicine, Nagoya University, 65 Tsaruama-cho Show-ku, Nagoya 466-8550, Japan.

Yoshimoto, T
Postgraduate Student, Research Students, Associate Professor, and Professor, respectively, Department of Legal Medicine and Bioethics, Postgraduate School of Medicine, Nagoya University, 65 Tsaruama-cho Show-ku, Nagoya 466-8550, Japan.

Nozawa, H
Associate Professor, Department of Legal Medicine, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431-3124, Japan.

Ohtaki, H
Postgraduate Student, Research Students, Associate Professor, and Professor, respectively, Department of Legal Medicine and Bioethics, Postgraduate School of Medicine, Nagoya University, 65 Tsaruama-cho Show-ku, Nagoya 466-8550, Japan.

Kato, Y
Research Student and Professor, respectively, Department of Legal Medicine, Showa University School of Medicine, 1-5-8 Hatnodai, Shinagawaku Tokyo 142-855, Japan.

Sato, K
Research Student and Professor, respectively, Department of Legal Medicine, Showa University School of Medicine, 1-5-8 Hatnodai, Shinagawaku Tokyo 142-855, Japan.

Yamamoto, T
Postgraduate Student, Research Students, Associate Professor, and Professor, respectively, Department of Legal Medicine and Bioethics, Postgraduate School of Medicine, Nagoya University, 65 Tsaruama-cho Show-ku, Nagoya 466-8550, Japan.

Tamaki, K
Lecturer, Department of Genetics, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK.

Katsumata, Y
Postgraduate Student, Research Students, Associate Professor, and Professor, respectively, Department of Legal Medicine and Bioethics, Postgraduate School of Medicine, Nagoya University, 65 Tsaruama-cho Show-ku, Nagoya 466-8550, Japan.


Abstract
We investigated the usefulness of a toothbrush as a source of DNA for an unidentified cadaver. Ten toothbrushes were obtained from ten individuals along with their peripheral blood. We recovered from 10 to 430 ng of DNA from all but one of the toothbrushes. All ten toothbrushes, including the one containing no detectable DNA by fluorometry, were typed correctly at all of the loci tested, including nine STRs. Three toothbrushes obtained in two actual deaths also identified two victims and one suspect. Therefore, toothbrushes seem to be useful as a source of evidential DNA for personal identification.

Keywords:
D7S8, DNA typing, forensic science, GC, GYPA, HBGG, HLA-DQA1, LDLR, polymerase chain reaction (PCR), short tandem repeats, toothbrush

Paper ID: JFS45327

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Author Tanaka M, Yoshimoto T, Nozawa H, Ohtaki H, Kato Y, Sato K, Yamamoto T, Tamaki K, Katsumata Y Title Usefulness of a toothbrush as a source of evidential DNA for typing Symposium , Committee on