An illustrated guide to RFLP troubleshooting
Benzinger, EA
Ohio Bureau of Criminal Identification and Investigation, London, OH.

Emerick, E
Ilinois State Police, Forensic Science Command, Springfield, IL.

Grigsby, N
Ilinois State Police, Forensic Science Command, Springfield, IL.

Lovekamp, ML
Ilinois State Police, Forensic Science Command, Springfield, IL.

Logeman, R
Ilinois State Police, Forensic Science Command, Springfield, IL.

Sallee, PJ
Ilinois State Police, Forensic Science Command, Springfield, IL.

Boster, KL
Ilinois State Police, Forensic Science Command, Springfield, IL.

Rehnstrom, A
Ilinois State Police, Forensic Science Command, Springfield, IL.

Reich, AK
Ilinois State Police, Forensic Science Command, Springfield, IL.

Small, AT
Ilinois State Police, Forensic Science Command, Springfield, IL.

Glasner, JA
Ilinois State Police, Forensic Science Command, Springfield, IL.

Coons, TM
Oregon State Police Forensic Laboratory, Portland, OR.

von Beroldingen, C
Oregon State Police Forensic Laboratory, Portland, OR.


Abstract
A large number of reagents and steps are required for restriction fragment length polymorphism (RFLP) analysis, which at times make determining the cause of any observed anomaly difficult. Troubleshooting problems in RFLP analysis is difficult and often the exact cause of a problem cannot be determined. In this paper a collection of controlled experiments detail the consequences of a number of human or materials problems. Although the focus is on forensic applications, this troubleshooting guide will be helpful to anyone employing Southern analysis.

Keywords:
DNA typing, forensic science, restriction fragment length polymorphism, Southern blot, troubleshooting

Paper ID: CH31665X

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Author Benzinger EA, Emerick E, Grigsby N, Lovekamp ML, Logeman R, Sallee PJ, Boster KL, Rehnstrom A, Reich AK, Small AT, Glasner JA, Coons TM, von Beroldingen C Title An illustrated guide to RFLP troubleshooting Symposium , Committee on