Anomalous Migration of PCR Products Using Nondenaturing Polyacrylamide Gel Electrophoresis: The Amelogenin Sex-Typing System
Eng, B
Technologist, DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton, Ontario, Canada.

Ainsworth, P
Director, DNA Diagnostic Laboratory, Victoria Hospital/Children's Hospital of Western Ontario, London, Ontario, Canada.

Waye, JS
Co-Director, DNA Diagnostic Laborato~, McMaster University Medical Centre; Assistant Professor, Department of Pathology, McMaster University, Hamilton, Ontario, Canada.


Abstract
Sex-typing of biological samples can be accomplished using the polymerase chain reaction (PCR) to amplify DNA sequences that are specific for the Y-chromosome. One such system is based on PCR amplification of the X-chromosome amelogenin gene and the amelogenin-like sequences located near the centromere of the Y-chromosome. The X and Y PCR products can be distinguished from each other on the basis of a 177 basepair (bp) insertion in the X relative to the Y. In this report, we demonstrate that the amelogenin PCR products migrate anomalously using non-denaturing polyacrylamide gel electrophoresis (ND-PAGE) as opposed to agarose gel electrophoresis or denaturing PAGE. These results may be relevant to the choice of electrophoretic system used to analyze highly polymorphic loci for individual identification.

Keywords:
amelogenin gene, electrophoretic anomalies, forensic science, genetic typing, nondenaturing PAGE, pathology and biology, polymerase chain reaction, polymerase chain reaction (PCR), sex-typing

Paper ID: JFS396941356

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Author Eng B, Ainsworth P, Waye JS Title Anomalous Migration of PCR Products Using Nondenaturing Polyacrylamide Gel Electrophoresis: The Amelogenin Sex-Typing System Symposium , Committee on