Effects of Cytosine Methylation at Restriction Sites on Deoxyribonucleic Acid (DNA) Typing
Washio, K
Research associate, Department of Legal Medicine, Institute of Community Medicine, University of Tsukuba. Ibaraki, Japan; also research fellow, Department of Anthropology, Faculty of Science, The University of Tokyo, Tokyo, Japan.

Ueda, S
Associate professor, Department of Anthropology, Faculty of Science, The University of Tokyo, Tokyo, Japan.

Misawa, S
Professor, Department of Legal Medicine, Institute of Community Medicine, University of Tsukuba, Ibaraki, Japan.


Abstract
The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcystosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.

Keywords:
deoxyribonucleic acid, deoxyribonucleic acid (DNA), forensic science, genetic typing

Paper ID: JFS356901277

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Author Washio K, Ueda S, Misawa S Title Effects of Cytosine Methylation at Restriction Sites on Deoxyribonucleic Acid (DNA) Typing Symposium , Committee on